LOCUS       pDONR/Zeo
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FEATURES             Location/Qualifiers
     misc_feature    complement(268..295)
                     /note="rrnB T2 transcription termination sequence (c)"
     misc_feature    complement(427..470)
                     /note="rrnB T1 transcription termination sequence (c)"
     primer_bind     537..552
                     /note="M13 Forward (-20) priming site"
     misc_recomb     570..668
                     /label=attL1
                     /note="attL1"
     misc_recomb     complement(2084..2180)
                     /label=attL2
                     /note="attL2 (c)"
     misc_signal     complement(2195..2214)
                     /note="T7 Promoter/priming site (c)"
     primer_bind     2222..2238
                     /note="M13 Reverse priming site"
     gene            2351..3160
                     /note="Kanamycin resistance gene"
     rep_origin      3281..3954
                     /note="pUC origin"
     vector          join(2092..3957,1..651)
                     /source="pDONR%99221"
                     /type="Donor Vector"
     misc_feature    669..691
                     /note="TEV site"
     source          692..2077
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /clone="MGC:61893 IMAGE:4593613"
                     /tissue_type="Kidney"
                     /clone_lib="NIH_MGC_75"
                     /lab_host="DH10B"
                     /note="Vector: pDNR-LIB"
     gene            692..2077
                     /gene="UGT1A9"
                     /gene_synonym=HLUGP4
                     /gene_synonym=LUGP4
                     /gene_synonym=UDPGT
                     /gene_synonym=UGT1AI
                     /db_xref="GeneID:54600"
                     /db_xref="HGNC:12541"
                     /db_xref="MIM:606434"
     CDS             692..2077
                     /dnas_title="UDP glucuronosyltransferase 1 family,
                     polypeptide A9"
                     /gene="UGT1A9"
                     /gene_synonym=HLUGP4
                     /gene_synonym=LUGP4
                     /gene_synonym=UDPGT
                     /gene_synonym=UGT1AI
                     /codon_start=1
                     /product="UDP glucuronosyltransferase 1 family,
                     polypeptide A9"
                     /protein_id="AAH58844.1"
                     /db_xref="GI:37590443"
                     /db_xref="GeneID:54600"
                     /db_xref="HGNC:12541"
                     /db_xref="MIM:606434"
                     /translation="MACTGWTSPLPLCVCLLLTCGFAEAGKLLVVPMDGSHWFTMRSV
                     VEKLILRGHEVVVVMPEVSWQLGRSLNCTVKTYSTSYTLEDLDREFKAFAHAQWKAQV
                     RSIYSLLMGSYNDIFDLFFSNCRSLFKDKKLVEYLKESSFDAVFLDPFDNCGLIVAKY
                     FSLPSVVFARGILCHYLEEGAQCPAPLSYVPRILLGFSDAMTFKERVRNHIMHLEEHL
                     LCHRFFKNALEIASEILQTPVTEYDLYSHTSIWLLRTDFVLDYPKPVMPNMIFIGGIN
                     CHQGKPLPMEFEAYINASGEHGIVVFSLGSMVSEIPEKKAMAIADALGKIPQTVLWRY
                     TGTRPSNLANNTILVKWLPQNDLLGHPMTRAFITHAGSHGVYESICNGVPMVMMPLFG
                     DQMDNAKRMETKGAGVTLNVLEMTSEDLENALKAVINDKSYKENIMRLSSLHKDRPVE
                     PLDLAVFWVEFVMRHKGAPHLRPAAHDLTWYQYHSLDVIGFLLAVVLTVAFITFKCCA
                     YGYRKCLGKKGRVKKAHKSKTH"
     misc_feature    692..2077
                     /note="UGT1A9 coding region"
ORIGIN      
          1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA
         61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA
        121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA
        181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC
        241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA
        301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC
        361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA
        421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG
        481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA
        541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC
        601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA
        661 AGCAGGCTct gaaaacttgt actttcaagg cgggaagcta ctggtagtgc ccatggatgg
        721 gagccactgg ttcaccatga ggtcggtggt ggagaaactc attctcaggg ggcatgaggt
        781 ggttgtagtc atgccagagg tgagttggca actgggaaga tcactgaatt gcacagtgaa
        841 gacttattca acttcatata ccctggagga tctggaccgg gagttcaagg cttttgccca
        901 tgctcaatgg aaagcacaag tacgaagtat atattctcta ttaatgggtt catacaatga
        961 catttttgac ttattttttt caaattgcag gagtttgttt aaagacaaaa aattagtaga
       1021 atacttaaag gagagttctt ttgatgcagt gtttctcgat ccttttgata actgtggctt
       1081 aattgttgcc aaatatttct ccctcccctc cgtggtcttc gccaggggaa tactttgcca
       1141 ctatcttgaa gaaggtgcac agtgccctgc tcctctttcc tatgtcccca gaattctctt
       1201 agggttctca gatgccatga ctttcaagga gagagtacgg aaccacatca tgcacttgga
       1261 ggaacattta ttatgccacc gttttttcaa aaatgcccta gaaatagcct ctgaaattct
       1321 ccaaacacct gttacggagt atgatctcta cagccacaca tcaatttggt tgttgcgaac
       1381 ggactttgtt ttggactatc ccaaacccgt gatgcccaac atgatcttca ttggtggtat
       1441 caactgccat cagggaaagc cgttgcctat ggaatttgaa gcctacatta atgcttctgg
       1501 agaacatgga attgtggttt tctctttggg atcaatggtc tcagaaattc cagagaagaa
       1561 agctatggca attgctgatg ctttgggcaa aatccctcag acagtcctgt ggcggtacac
       1621 tggaacccga ccatcgaatc ttgcgaacaa cacgatactt gttaagtggc taccccaaaa
       1681 cgatctgctt ggtcacccga tgacccgtgc ctttatcacc catgctggtt cccatggtgt
       1741 ttatgaaagc atatgcaatg gcgttcccat ggtgatgatg cccttgtttg gtgatcagat
       1801 ggacaatgca aagcgcatgg agactaaggg agctggagtg accctgaatg ttctggaaat
       1861 gacttctgaa gatttagaaa atgctctaaa agcagtcatc aatgacaaaa gttacaagga
       1921 gaacatcatg cgcctctcca gccttcacaa ggaccgcccg gtggagccgc tggacctggc
       1981 cgtgttctgg gtggagtttg tgatgaggca caagggcgcg ccacacctgc gccccgcagc
       2041 ccacgacctc acctggtacc agtaccattc cttggacTAG GACCCAGCTT TCTTGTACAA
       2101 AGTTGGCATT ATAAGAAAGC ATTGCTTATC AATTTGTTGC AACGAACAGG TCACTATCAG
       2161 TCAAAATAAA ATCATTATTT GCCATCCAGC TGATATCCCC TATAGTGAGT CGTATTACAT
       2221 GGTCATAGCT GTTTCCTGGC AGCTCTGGCC CGTGTCTCAA AATCTCTGAT GTTACATTGC
       2281 ACAAGATAAA ATAATATCAT CATGAACAAT AAAACTGTCT GCTTACATAA ACAGTAATAC
       2341 AAGGGGTGTT ATGAGCCATA TTCAACGGGA AACGTCGAGG CCGCGATTAA ATTCCAACAT
       2401 GGATGCTGAT TTATATGGGT ATAAATGGGC TCGCGATAAT GTCGGGCAAT CAGGTGCGAC
       2461 AATCTATCGC TTGTATGGGA AGCCCGATGC GCCAGAGTTG TTTCTGAAAC ATGGCAAAGG
       2521 TAGCGTTGCC AATGATGTTA CAGATGAGAT GGTCAGACTA AACTGGCTGA CGGAATTTAT
       2581 GCCTCTTCCG ACCATCAAGC ATTTTATCCG TACTCCTGAT GATGCATGGT TACTCACCAC
       2641 TGCGATCCCC GGAAAAACAG CATTCCAGGT ATTAGAAGAA TATCCTGATT CAGGTGAAAA
       2701 TATTGTTGAT GCGCTGGCAG TGTTCCTGCG CCGGTTGCAT TCGATTCCTG TTTGTAATTG
       2761 TCCTTTTAAC AGCGATCGCG TATTTCGTCT CGCTCAGGCG CAATCACGAA TGAATAACGG
       2821 TTTGGTTGAT GCGAGTGATT TTGATGACGA GCGTAATGGC TGGCCTGTTG AACAAGTCTG
       2881 GAAAGAAATG CATAAACTTT TGCCATTCTC ACCGGATTCA GTCGTCACTC ATGGTGATTT
       2941 CTCACTTGAT AACCTTATTT TTGACGAGGG GAAATTAATA GGTTGTATTG ATGTTGGACG
       3001 AGTCGGAATC GCAGACCGAT ACCAGGATCT TGCCATCCTA TGGAACTGCC TCGGTGAGTT
       3061 TTCTCCTTCA TTACAGAAAC GGCTTTTTCA AAAATATGGT ATTGATAATC CTGATATGAA
       3121 TAAATTGCAG TTTCATTTGA TGCTCGATGA GTTTTTCTAA TCAGAATTGG TTAATTGGTT
       3181 GTAACACTGG CAGAGCATTA CGCTGACTTG ACGGGACGGC GCAAGCTCAT GACCAAAATC
       3241 CCTTAACGTG AGTTACGCGT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG
       3301 ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC
       3361 GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC
       3421 TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTTCTTCTA GTGTAGCCGT AGTTAGGCCA
       3481 CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT
       3541 GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC
       3601 GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG
       3661 AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC
       3721 CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC
       3781 GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT
       3841 CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC
       3901 CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTT
//
